 =Rhizoglyphus robini

 PoloPlus  Version 2.0
 Date: 30 APR 2026
 Rhizoglyphus robini                                                     
 Data file: C:\Users\Hosein\Desktop\Pro+Nickle.txt
 Number of preparations: 2
 Number of dose groups: 32
 Model: Probit
 Natural Response Parameter: no
 Convert doses to logarithms: yes
 LDs:  50
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 Rhizoglyphus robini                                                     
  
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 Intercepts and slopes unconstrained. Preparation is ( 1) Propar  
 Not estimating natural response
  
             parameter   standard error   t ratio
 Propar       -3.072          0.310       -9.900
 SLOPE         2.065          0.201       10.286
  
 Variance-Covariance matrix
                 Propar        SLOPE       
   Propar      0.962652E-01 -0.608934E-01
   SLOPE      -0.608934E-01  0.403035E-01
  
 Chi-squared goodness of fit test
 prep             dose      n       r   expected  residual   probab  std resid
 Propar        100.000     28.     23.     23.94    -0.942    0.855    -0.506
               100.000     30.     26.     25.65     0.348    0.855     0.180
               100.000     32.     27.     27.36    -0.362    0.855    -0.182
               100.000     21.     18.     17.96     0.043    0.855     0.027
                50.000     22.     15.     14.72     0.285    0.669     0.129
                50.000     26.     18.     17.39     0.609    0.669     0.254
                50.000     19.     13.     12.71     0.291    0.669     0.142
                50.000     29.     20.     19.40     0.603    0.669     0.238
                25.000     24.     10.     10.24    -0.240    0.427    -0.099
                25.000     31.     13.     13.23    -0.227    0.427    -0.083
                25.000     19.      8.      8.11    -0.107    0.427    -0.050
                25.000     35.     15.     14.93     0.066    0.427     0.023
                12.500     32.      7.      6.72     0.280    0.210     0.122
                12.500     25.      5.      5.25    -0.250    0.210    -0.123
                12.500     38.      8.      7.98     0.020    0.210     0.008
                12.500     30.      6.      6.30    -0.300    0.210    -0.134
  
 chi-square: 0.548     degrees of freedom: 14     heterogeneity: 0.039
  
 Effective Doses
                   dose      limits    0.90     0.95     0.99
 LD50  Propar    30.721      lower   27.164   26.501   25.207
                             upper   34.646   35.469   37.173
  
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 Intercepts and slopes unconstrained. Preparation is ( 2) Pro.Nick
 Not estimating natural response
  
             parameter   standard error   t ratio
 Pro.Nick     -2.880          0.275      -10.458
 SLOPE         2.306          0.211       10.937
  
 Variance-Covariance matrix
                 Pro.Nick      SLOPE       
   Pro.Nick    0.758360E-01 -0.563276E-01
   SLOPE      -0.563276E-01  0.444560E-01
  
 Chi-squared goodness of fit test
 prep             dose      n       r   expected  residual   probab  std resid
 Pro.Nick       60.000     26.     23.     23.11    -0.110    0.889    -0.069
                60.000     29.     26.     25.78     0.223    0.889     0.132
                60.000     30.     27.     26.67     0.334    0.889     0.194
                60.000     20.     18.     17.78     0.223    0.889     0.158
                30.000     23.     15.     16.12    -1.116    0.701    -0.508
                30.000     24.     18.     16.82     1.184    0.701     0.528
                30.000     20.     13.     14.01    -1.014    0.701    -0.495
                30.000     28.     20.     19.62     0.381    0.701     0.157
                15.000     25.     10.     10.83    -0.834    0.433    -0.337
                15.000     30.     13.     13.00    -0.001    0.433     0.000
                15.000     20.      8.      8.67    -0.667    0.433    -0.301
                15.000     34.     15.     14.73     0.266    0.433     0.092
                 7.500     35.      7.      6.80     0.198    0.194     0.084
                 7.500     28.      5.      5.44    -0.442    0.194    -0.211
                 7.500     34.      8.      6.61     1.392    0.194     0.603
                 7.500     32.      6.      6.22    -0.219    0.194    -0.098
  
 chi-square: 1.529     degrees of freedom: 14     heterogeneity: 0.109
  
 Effective Doses
                   dose      limits    0.90     0.95     0.99
 LD50  Pro.Nick  17.736      lower   15.854   15.503   14.820
                             upper   19.804   20.235   21.123
  
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 HYPOTHESIS OF EQUALITY (equal slopes, equal intercepts): REJECTED (P<0.05)
    (chi-square: 29.24,  degrees of freedom: 2,  tail probability: 0.000)
  
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 HYPOTHESIS OF PARALLELISM (equal slopes): NOT REJECTED (P>0.05)
    (chi-square: 0.69,  degrees of freedom: 1,  tail probability: 0.408)
  
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 Lethal dose ratio (LD50)
              ratio      limits     0.95
 Pro.Nick     1.732      lower     1.421
                         upper     2.111
  
